0032 Polymorphonuclear leukocytes phagocytic capacity in workers occupationally exposed to benzene
研究了54名油漆厂工人，通过尿中S-苯基巯基尿酸（SPMA）评估苯暴露水平，分析多形核白细胞的吞噬能力（NBT还原、过氧化氢产生、细胞黏附），发现暴露组与对照组无显著差异，但三个参数随SPMA浓度升高呈下降趋势。
<h3>Objectives</h3> To determine phagocytic capacity of PMN leukocytes in workers occupationally exposed to benzene. <h3>Method</h3> Cross-sectional study that included 54 workers of a paint manufacture company in Mexico City; exposure to benzene was determined through S-phenylmercapturic acid (SPMA) presence in urine. The PMN phagocytic capacity analysis included three parameters: 1) nitro-blue tetrazolium (NBT) reduction, 2) hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production, and 3) cell adhesion (CAD) <h3>Results</h3> In the whole of workers included in the study, NBT reduction = 0.419 ± 0.075, H<sub>2</sub>O<sub>2</sub> production = 6.7 ± 1.4 ng, and CAD = 58.3 ± 6.2 μg. SPMA was identified in all workers although 24 of them are not in occupationally exposure to organic solvents (2.3 ± 0.81 μmol/mol creatinine), while the remaining 30 handle these substances (3.2 ± 1.8, <i>p = 0</i>.02). Among these exposure groups, there were not statistically differences in any of the parameters analysed. Although the simple regression analysis of these parameters with the concentration of SPMA identified in urine, a decrease was observed in NBT reduction (β= -0.009, R<sup>2</sup>= 0.01), in H<sub>2</sub>O<sub>2</sub> production (β= -0.16, R<sup>2</sup>= 0.02), and in CAD (β= -0.53, R<sup>2</sup>= 0.01), none was statistically significant (<i>p ≥ 0</i>.05) <h3>Conclusions</h3> PMN phagocytic capacity in the workers studied seems to be intact. Attract attention the consistently decrease of the three parameters in relation to the concentration of SPMA identified in urine even when there was no statistical significance. Some limitations do not allow a more complete analysis, so it is encouraged to make further studies.